HT29 gluc C1

产品说明

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

培养基

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质