MANIKA human cell line

产品说明

一般描述

Consanguineous: Yes

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

A: 301
Cw: 602
B: 5001
HLA DRA: 101
HLA DRB1: 701
HLA DRB4: 101
HLA DQA1: 201
HLA DQB1: 0201 03032
HLA DPA1: 01 0201
HLA DPB1: 0401 1301

血清学分析

HLA A: 3
HLA A ief: 3.2
HLA C: 6
HLA B: 50
HLA B ief: 50.2
Bw4/bw6: 6
HLA DR: 7
HLA DR53: 53
HLA DQ: 2 3

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 ℃. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

产品性质