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ML-1

货号
CB_88113007
规格纯度
88113007
参考价格
4474.8 *本价格含增值税费
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数量
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产品名称:
ML-1
产品介绍:

产品说明

细胞系来源

Human acute myeloblastic leukaemia

细胞系描述

ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

应用

ML-1 has been used to compare the cell response to mafosfamide cyclohexylamine salt, 4-hydro-peroxy-cyclophosphamide and β-D-glucose-isophosphoramide mustard, which are oxazaphosphorine agents.

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,10
vWA: 16

培养基

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

传代培养常规

Maintain cultures between 2-9 x100,000 cells/ml; 5% CO2; 37℃. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源human blood
生长模式Suspension
核型Not specified
形态学Lymphoblastoid
产品Not specified
受体Not specified
technique(s)cell culture | mammalian: suitable
相关疾病cancer
运输dry ice
储存温度−196℃

Sigma-Aldrich

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