产品说明
细胞系来源
Human ovarian cancer; oestrogen receptor negative
DNA图谱分析
STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 11,12
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 15,16
培养基
RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. Doubling time approximately 120 hours.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
生物来源 | human ovary |
生长模式 | Adherent |
核型 | For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72. |
形态学 | Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres. |
产品 | Not specified |
受体 | Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72. |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
Sigma-Aldrich