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3A(tPA-30-1)

货号
CB_96020926
规格纯度
96020926
参考价格
4633 *本价格含增值税费
促销
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数量
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产品名称:
3A(tPA-30-1)
产品介绍:

产品说明

细胞系来源

Human placenta, SV40 transformed

细胞系描述

Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1

培养基

α-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36℃. Change medium 2-3 times weekly.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质

生物来源human placenta
生长模式Adherent
核型Hypodiploid, modal chromosome number of 42 occuring in 34% of cells. Most cells contained 41-45 chromosomes. Cells with 44 chromosomes also occurred at a high frequency (26%). Cells having a higher ploidly occurred at 20.7%, which is very high.
形态学Epithelial
产品At 40C cells synthesize human chorionic gonadotrophin (hCG) and alkaline phosphatase.
受体Not specified
technique(s)cell culture | mammalian: suitable
相关疾病cancer
运输dry ice
储存温度−196℃

Sigma-Aldrich

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