产品说明
细胞系来源
Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage D
细胞系描述
The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes′ stage D
DNA图谱分析
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17
培养基
Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
生物来源 | human colon |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Epithelial |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |