产品说明
细胞系来源
Human lymphoblastoid, normal,EBV-transformed
细胞系描述
Derived from mononuclear cells from peripheral blood of a 49 year old normal male. Cells were used as controls for cellular radiosensitivity studies and analysis of cell survival after UV-irradiation. The line LB173 has been established by subsequent transformation with EBV. Fibroblasts from the same individual have been designated 144BR.
应用
Mutation studies
培养基
RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
传代培养常规
Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37℃. Cells form floating aggregates.
其他说明
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产品性质
生物来源 | human blood |
生长模式 | Suspension |
核型 | Not specified |
形态学 | Lymphoblastoid |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |