PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

产品说明

一般描述

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³².
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³² in HEK293 cells.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α
Human

包装

50 μg in Plastic ampoule

应用

Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
HEK293 cells

其他说明

This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息