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NovaBlue Singles Competent Cells-Novagen

品牌
Sigma-Aldrich
货号
70181-M
规格纯度
NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.
参考价格
2823.5 *本价格含增值税费
促销
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数量
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产品名称:
NovaBlue Singles Competent Cells-Novagen
产品介绍:

产品说明

一般描述

Genotype:

endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
NovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency,facilitates plasmid stability, and allows blue/whitescreening with appropriate plasmids.

NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-l volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37℃ for 30 minutes (when selecting for kanamycin resistance).
NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

组分

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

DNA图谱分析

Genotype: endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
manufacturer/tradenameNovagen®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
细胞转化transformation efficiency: >1.5×108 cfu/μg
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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