产品说明
一般描述
Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.
应用
Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.
包装
500, 1000, 2500 units in glass bottle
生化/生理作用
Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.
单位定义
One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 ℃.
外形
Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5
分析说明
Protein determined by biuret.
基本信息
| 酶学委员会(EC)编号 | 4.4.1.5 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00131225 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 类型 | Grade IV |
| 形式 | buffered aqueous glycerol solution |
| specific activity | ≥400 units/mg protein |
| 异质活性 | glyoxalase II ≤1% |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
