产品说明
一般描述
A potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription.
A potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC50 = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks cellular expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription. A 50 mM (5 mg/454 µl) solution of JNK Inhibitor II (Cat. No. 420128) in DMSO is also available.
包装
5, 25, 50 mg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Target IC50: 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3
Primary Target
JNK 1, JNK 2, JNK 3
Reversible: yes
Cell permeable: yes
Product competes with ATP.
警告
Toxicity: Standard Handling (A)
制备说明
For every 10 µM concentration of JNK Inhibitor II, include 0.1% DMSO in the culture medium. Pre-warming of culture medium and addition of BSA to the medium may enhance its solubility.
重悬
Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.
其他说明
Shin, M., and Boyd, Y.D. 2002. Biochim. Biophys. Acta1589, 311.
Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA98, 13681.
Han, Z., et al. 2001. J. Clin. Invest.108, 73.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C14H8N2O |
分子量 | 220.23 |
产品性质
质量水平 | 200 |
测定 | ≥98% (HPLC) |
形式 | solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze protect from light |
颜色 | yellow-orange |
溶解性 | DMSO: 15 mg/mL |
运输 | ambient |
储存温度 | −20℃ |
InChI | 1S/C14H8N2O/c17-14-9-5-2-1-4-8(9)13-12-10(14)6-3-7-11(12)15-16-13/h1-7H,(H,15,16) |
InChI key | ACPOUJIDANTYHO-UHFFFAOYSA-N |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich