产品说明
应用
HiCrome™ ECC 选择性琼脂是一种选择性培养基，推荐用于对水和食物样品中的大肠杆菌和大肠菌群进行同时检测。该培养基中的成分甚至有助于亚致死性损伤的大肠菌迅速生长，Tergitol™ 可以抑制革兰氏阳性和某些革兰氏阴性细菌，而不影响大肠菌。显色混合物包含两种显色底物：Salmon-GAL 和 X-葡萄糖苷酸。大肠菌产生的 β-D-半乳糖苷酶可裂解 Salmon-GAL，使大肠菌菌落呈现鲜肉色至红色。大肠杆菌产生的 β-D-葡萄糖醛酸苷酶则可裂解 X-葡萄糖苷酸。由于 Salmon-GAL 和 X-葡萄糖苷酸均被裂解，因此大肠杆菌形成深蓝色到紫色的菌落。添加色氨酸可增进吲哚反应。为了确认大肠杆菌，可将一滴柯氏试剂(目录号 60983)滴在深蓝色到紫色的菌落上。形成樱桃红色则说明反应呈阳性。
制备说明
Suspend 26.5 g in 1 litre distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species add 5 mg Cefsulodin (Cat. No. 22126) for surface and pour plate method and 10 mg Cefsulodin for membrane filter technique. Mix well.
重悬
Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TERGITOL is a trademark of The Dow Chemical Company ("Dow") or an affiliated company of Dow
基本信息
| MDL编号 | MFCD01634645 |
| NACRES | NA.85 |
产品性质
| 质量水平 | 200 |
| 无菌性 | non-sterile |
| 形式 | powder |
| 保质期 | limited shelf life, expiry date on the label |
| 组成 | agar, 10.0 g/L casein enzymic hydrolysate, 3.3 g/L chromogenic mixture, 0.43 g/L disodium hydrogen phosphate, 1 g/L peptone, special, 6.0 g/L sodium chloride, 2 g/L sodium dihydrogen phosphate, 0.6 g/L sodium pyruvate, 1 g/L sorbitol, 1 g/L Tergitol™ 7, 0.15 g/L tryptophan, 1 g/L |
| manufacturer/tradename | NutriSelect® Plus |
| 最终pH | 6.8±0.2 (25 ℃) |
| application(s) | agriculture bioburden testing environmental food and beverages water monitoring microbiology |
| 适用性 | selective and differential for Citrobacter spp. selective and differential for Enterobacter spp. selective and differential for Escherichia coli selective and differential for Salmonella spp. selective and differential for Shigella spp. selective and differential for coliforms selective and differential for enterobacteriaceae |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 |
| 预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| 危险分类 | Skin Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
