产品说明
特异性
Serotonin (5-Hydroxytryptamine)
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
免疫原
5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
应用
Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Research Category
Neuroscience
Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurotransmitters & Receptors
外形
Rabbit antiserum. Liquid with 0.05% sodium azide.
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry: suitable |
运输 | dry ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich