产品说明
一般描述
Alpha B-crystallin is a major structural protein in the vertebrate eye lens, but is also found in cardiac and skeletal muscles, kidney, and brain. Alpha B-crystallin is also found to be a component of Rosenthal fibers and a consistent tissue marker of epileptic foci. It occurs as large aggregates of up to 106 Da, composed of two proteins, αA- and αB-crystallin. The αA gene encodes a polypeptide of 173 residues, while αB-crystallin gene encodes a 175 residue polypeptide. αB-crystallin, like the small heat shock proteins (sHsp′s), is induced by heat or osmotic shock and is upregulated by stress. α-crystallin and the sHsp′s protect other proteins from heat-induced aggregation. The two other crystallin families, β and ɣ, are homologous to each other but not to the α-family or the sHsp′s.
特异性
This antibody recognizes the N-terminus of alpha B-crystallin.
免疫原
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus human alpha B-crystallin.
应用
This Anti-alpha B-crystallin Antibody is validated for use in WB, IH(P) for the detection of alpha B-crystallin.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in rat lens fiber tissue. A 1:2,000 dilution from a representative lot detected Alpha-crystallin B chain in strong rat lens fiber tissue (data not provided). A 1:1,000 dilution from a representative lot detected Alpha-crystallin B chain in strong muscle fiber tissue (data also not provided). It is recommended to use a 1:2,000 dilution only for strong rat lens fiber tissue.
Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of human kidney and calf kidney tissue lysate.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
质量
Evaluted by Western Blot in bovine calf lens tissue lysate.
Western Blot analysis: 0.05 µg/mL of this antibody detected Alpha-crystallin B chain in 10 µg of bovine calf lens tissue lysate.
目标描述
~20 kDa observed. Uncharacterized bands at ~37 and 43 kDa may be observed in some cell lyates.
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Bovine calf lens, human kidney and calf kidney tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | bovine, rat, human |
species reactivity (predicted by homology) | horse (based on 100% sequence homology), mouse (based on 100% sequence homology), ox (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology) |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
NCBI登记号 | NP_001876 |
UniProt登记号 | P02511 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich