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Anti-EBF-3 Antibody

品牌
Sigma-Aldrich
货号
AB10525
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6022.61 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-EBF-3 Antibody
Transcription factor COE3, Early B-cell factor 3, EBF-3, Olf-1/EBF-like 2, O/E-2, OE-2
产品介绍:

产品说明

一般描述

The family of Early B-cell Factor (EBF) or Collier/Olf-1/EBF (COE) proteins, EBF1, EBF2, and EBF3, are ubiquitous zinc-binding transcription factors found in many species, including human, Drosophila melanogaster, and Caenorhabditis elegans. EBF proteins are capable of forming homo- and heterodimers that bind to DNA at specific sites that include the sequence 5′-ATTCCCNNGGGAATT-3′. EBF1, the prototypical member of this family, may employ epigenetic mechanisms, such as DNA methylation and chromatin remodeling, to regulate genes involved in B-cell development such as Pax5. The second member of this family EBF2 is involved in bone development, adipogenesis, and CNS development, processes which may also hinge on EBF1. EBF3 has been characterized as a tumor suppressor protein, due to its role in regulating multiple genes such as those for cyclins and CDKs which control cell growth, differentiation, and apoptosis. However, emerging evidence indicates that all EBF proteins may play a role in the development of various cancers.

特异性

This antibody recognizes EB-3 but not EB-1 or EB-2.

免疫原

KLH-conjugated linear peptide corresponding to mouse EBF-3.

应用

Western Blot Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF-3. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected EBF-3 in mouse cryosections of wild type spinal cord tissue. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected EBF-3 in COS-7 cells transfected with EBF expressing plasmids. (Image courtesy of Dr. Giacomo Consalez, San Raffaele Scientific Institute.)
Dot Blot Analysis: EBF-1, EBF-2, and EBF-3 peptides from a representative lot were probed with Anti-EBF-3 (1:100 dilution). No cross reactivity to peptides for EBF-1 & EBF-2 were observed.
Anti-EBF-3 Antibody is an antibody against EBF-3 for use in Western Blotting, IHC(P), Immunofluorescence, Dot Blot.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

质量

Evaluated by Western Blot in mouse E16 brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected EBF-3 in 10 µg of mouse E16 brain tissue lysate.

目标描述

~72 kDa observed. An uncharacterized band may appear at ~42, ~55, and ~108 kDa in some lysates.

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse E16 brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse
species reactivity (predicted by homology)human (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号NP_001106886
UniProt登记号O08791
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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