产品说明
一般描述
Monoclonal Anti-Coilin (mouse IgG1 isotype) is derived from the pδ hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with coilin. Coilin contains two nuclear localization sequences (NLS) (at amino acid 107-112 and 181-198) and several serine residues that are phosphorylated in vivo. The description of specific intranuclear structures known today as Cajal bodies was first published in 1903 by the neuro-cytologist Ramon-γ-Cajal who discovered that neurons contained spherical structures of around 0.5 μm in diameter that were often associated with nucleoli, nucleolar accessory bodies. It was found that patients with auto-antibodies against coiled bodies recognize a protein of 80 kDa termed p80-coilin. Nuclear antigens shown to colocalize with p80 coilin in Cajal bodies include basal transcription factors, cell cycle factors (cdks), splicing snRNPs and nucleolar factors including snoRNP.
特异性
The antibody recognizes the C-terminal region of human coilin and does not recognize mouse coilin.
免疫原
C-terminal (389 amino acids) human coilin
应用
Monoclonal Anti-Coilin antibody produced in mouse has been used in:
生化/生理作用
Mutating Serine-202 to Aspartate causes the disappearance of coiled bodies and a redistribution of coilin to intranucleolar domains. Coilin plays a key role in ribonucleoprotein and Cajal body formation.
外形
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD03792415 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | pδ, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~80 kDa by SDS-PAGE |
| species reactivity | human |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunocytochemistry: suitable microarray: suitable western blot: 1-2 μg/mL using HeLa nuclear extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P38432 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... COIL(8161) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
