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Monoclonal Anti-Caspase 12 antibody produced in rat

品牌
Sigma-Aldrich
货号
C7611
规格纯度
clone 14F7, purified immunoglobulin, buffered aqueous solution
参考价格
5688.25 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Monoclonal Anti-Caspase 12 antibody produced in rat
产品介绍:

产品说明

一般描述

Caspase 12 is a protein that belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The human caspase 12 gene is localized at a single locus within the caspase 1/ICE gene cluster on chromosome 11q22. The functionality is lost in humans and they cannot play a role in Alzheimer′s disease. Caspase-12 is detected in endoplasmic reticulum (ER), plays a role in ER-mediated apoptosis and inflammatory caspase activation pathway.
Monoclonal Anti-Caspase 12 (rat IgG2a isotype) is derived from the 14F7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from CD rats immunized with a recombinant mouse caspase 12. Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspase 12 is ubiquitously expressed in mouse tissues, at high levels in muscle, liver and kidney and at moderate levels in brain, where it is present in cortical neurons, Purkinje cells, brainstem neurons, and olfactory neurons.

特异性

Recognizes full length and possibly truncated p20 caspase 12.

免疫原

recombinant mouse caspase 12 (residues 95-318).

应用

Monoclonal Anti-Caspase 12 has been used in immunoblotting and immunocytochemistry (4% paraformaldehyde-fixed, paraffin-embedded).

生化/生理作用

Murine caspase 12 (CASP12) is activated during endoplasmic reticulum stress and activates other caspases which act on substrates, ultimately leading to apoptosis. The protein has been shown to be activated during progression of retinal degeneration in animal models. In mice models, CASP12 has been shown to cause neuronal death resulting from ischemia/reperfusion. Casp12 might stimulate nuclear factor-κB (NF-κB) by directing the post-translational degradation of inhibitor of kappa B (IκBα). Overexpression of caspase 12 induces apoptosis, which is inhibited by the pan caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethyl ketone (z-VAD-FMK).
Caspase 12 (CASP12) has a downregulatory function during infection and may play a role in protection against inflammatory disease. It is associated with rheumatoid arthritis in African-Americans. Caspase-12 inhibits caspase-1 activation and is associated with susceptibility factor for bacterial sepsis but does not influence the susceptibility to Candida sepsis.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

制备说明

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD03454855
NACRESNA.41

产品性质

质量水平200
生物来源rat
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆14F7, monoclonal
形式buffered aqueous solution
分子量antigen ~50 kDa
species reactivityrat, mouse
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells
同位素/亚型IgG2a
UniProt登记号Q920D5
运输dry ice
储存温度−20℃
Gene Informationmouse ... Casp12(12364)
rat ... Casp12(156117)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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