您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-AP Endonuclease antibody, Mouse monoclonal

Anti-AP Endonuclease antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
A2105
规格纯度
clone APEREF, purified from hybridoma cell culture
参考价格
4731.57 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-AP Endonuclease antibody, Mouse monoclonal
Anti-APEN
Anti-APEX1
Monoclonal Anti-AP Endonuclease antibody produced in mouse
Anti-HAP1
Anti-Apurinic/Apyrimidinic Endonuclease
Anti-APE/Ref1
Anti-AP lysase
Anti-APE1
Anti-APEX nuclease
产品介绍:

产品说明

一般描述

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) is a crucial enzyme in the DNA base excision repair (BER) pathway. It recognizes baseless sites in the DNA following spontaneous base loss or removal of a damaged base by different DNA glycosylases. This enzyme is the major AP endonuclease in mammalian cells. The human enzyme shows a high degree of homology with other mammalian AP endonucleases (bovine, mouse, rat, and hamster). APE/Ref1 is a multifunctional enzyme. Its N-terminal region includes a nuclear localization sequence and a redox activity zone, while its AP endonuclease activity resides in the C-terminal region. Redox-factor 1 (Ref1) refers to the reduction/oxidation function of the enzyme.
Monoclonal Anti-AP Endonuclease recognizes human, canine, rat, and mouse AP endonuclease (approx. 37kDa).
The AP endonuclease is a DNA damage repair gene involved in base excision repair.

免疫原

recombinant human AP endonuclease.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-AP endonuclease antibodies have been used for western blot analysis. The antibody can also be used for microarray, indirect ELISA and immunocytochemistry assays.

生化/生理作用

AP endonuclease (APE/Ref1 or Apurinic/apyrimidinic endonuclease) has a 3′-repair diesterase or phosphatase activity. The major DNA repair activity of the enzyme is nicking the DNA phosphodiester backbone 5′ to the AP site leaving a 3′-hydroxyl group and a 5′-deoxyribose phosphate. APE/Ref1 stimulates the DNA binding activity of many transcription factors (Fos, Jun, NF?B, PAX, HIF1, p53 and CREB), which are involved in cancer promotion and progression. The protein was found at elevated levels in several tumors (ovarian, prostate, cervical and germ cells). Redox-factor 1 (Ref1) maintains transcription factors in their active reduced states.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01633033
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆APEREF, monoclonal
形式buffered aqueous solution
分子量antigen ~37 kDa
species reactivityrat, human, canine, mouse
浓度~2 mg/mL
technique(s)immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of Raji cells
同位素/亚型IgG1
UniProt登记号P27695
运输dry ice
储存温度−20℃
Gene Informationhuman ... APEX1(328)
mouse ... Apex1(11792)
rat ... Apex1(79116)

Sigma-Aldrich

推荐产品