产品说明
一般描述
Palladin is a phosphoprotein, which is widely expressed in vertebrate cells and tissues. It is expressed as three major isoforms of 90, 140, and 200 kDa. Palladin consists of a proline-rich region in the NH2-terminal half of the protein and three tandem Ig C2 domains in the COOH-terminal.
Palladin exists in humans as 7 different isoforms and is a component of actin-containing microfilaments.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer.
Rabbit anti-Palladin antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide.
应用
Rabbit anti-palladin antibody can be used for immunoprecipitation (5-10μg), indirect immunofluorescence (2-5μg/mL) and western blot (0.5-1.0μg/mL) using HeLa cells.
生化/生理作用
Palladin controls cell shape, adhesion, and contraction. It interacts with the actin-associated proteins α-actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. The Ig3 domain of palladin binds to F-actin. Its expression is required for normal mammalian embryogenesis since its inactivation leads to embryonic lethality.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~140/90 kDa |
| species reactivity | human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of HeLa cells indirect immunofluorescence: 2-5 μg/mL using human HeLa cells western blot: 0.5-1.0 μg/mL using whole extracts of human HeLa cells |
| UniProt登记号 | Q8WX93 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PALLD(23022) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
