Anti-PRDM14 Antibody

产品说明

一般描述

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266 - 367) and six zinc finger repeats (aa 400 - 568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Recently, PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in embryonic stem cells

特异性

Human

免疫原

Human recombinant PRDM14.
Epitope: N-terminus

应用

Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research
This Anti-PRDM14 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of PRDM14 .
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (CHAN et al., 2013. STEM CELLS, 31:682–692)
ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468)

质量

Evaluated by Western Blotting in Human ES cell cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM14 in undifferentiated human ES cell lysate.

目标描述

~64 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Unpurified
Raw rabbit polyclonal serum
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息