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Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
E3906
规格纯度
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
5045.46 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit
Anti-EEA1
Anti-Zinc Finger FYVE Domain-containing Protein 2
Anti-Endosome-associated Protein p162
产品介绍:

产品说明

一般描述

Anti-Early Endosomal Antigen 1 (C-terminal), is produced in rabbit using a synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to keyhole limpet hemocyanin (KLH), as immunogen. Early Endosomal Antigen 1 (EEA1) is a 162 kDa autoantigen, which encodes for around 1400 amino acid proteins. EEA1 is a dimer, which comprises extensive coiled-coil regions. At its C-terminus, it contains a cysteine-rich zinc-finger-like domain named FYVE domain. The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins

免疫原

synthetic peptide corresponding to amino acid residues 1391-1410 of human EEA1, conjugated to KLH. The corresponding sequence is identical in mouse.

应用

Anti-Early Endosomal Antigen 1 (C-terminal) antibody produced in rabbit has been used in:

  • fluorescent staining
  • confocal laser scanning microscopy
  • immunoprecipitation
  • indirect immunofluorescence microscopy
  • fluorescence microscopy
  • immunoblotting
  • immunofluorescence.

生化/生理作用

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic side of early endosomes. EEA1 controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population.
Anti- Early Endosomal Antigen 1 (EEA1) may be used as an early endosome marker. The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. It is associated with subacute systemic lupus erythematosus that specifically localized to early endosomes.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01633310
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~160 kDa
species reactivityhuman, rat, mouse
浓度~1 mg/mL
technique(s)indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of human HeLa cells
western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of mouse NIH3T3 cells
UniProt登记号Q15075
运输dry ice
储存温度−20℃
Gene Informationhuman ... EEA1(8411)
mouse ... Eea1(216238)
rat ... Eea1(314764)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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