产品说明
一般描述
Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the HDAC1-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human and mouse histone deacetylase 1 (HDAC1).
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.
应用
Anti-Histone Deacetylase 1 (HDAC1) antibody, Mouse monoclonal has been used in:
生化/生理作用
HDAC1 is widely studied and is shown to actively modulate the eukaryotic chromatin structure. It deacetylyses lysine residues on core histones (H2A, H2B, H3 and H4) at the N-terminal. It is a vital component of cofactor complexes and is involved in transcription regulation. Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing.
外形
solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD01633420 |
NACRES | NA.41 |
产品性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | HDAC1-21, monoclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~65 kDa |
species reactivity | mouse, human |
浓度 | ~2 mg/mL |
technique(s) | immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 2-4 μg/mL using total cell extracts of HeLa cells |
同位素/亚型 | IgG3 |
UniProt登记号 | Q13547 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... HDAC1(3065) mouse ... Hdac1(433759) |
Sigma-Aldrich