产品说明
特异性
Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.
免疫原
Epitope: N-terminus, aa3-16 hBax protein
Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.
应用
Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).
Research Sub Category
BCL2 & Inhibition
Research Category
Apoptosis & Cancer
Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)
Immunofluorescence
Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)
Optimal working dilutions must be determined by end user.
联系
Replaces: 04-434
外形
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
Format: Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 2D2, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | immunofluorescence: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_004324.3 NM_138761.2 NM_138763.2 NM_138764.2 NM_138765.2 |
UniProt登记号 | Q07814 |
运输 | wet ice |
Gene Information | human ... BAX(581) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich