产品说明
一般描述
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
特异性
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle. It has been reported that this antibody does not react with GAPDH from E. coli.
免疫原
Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
应用
Research Category
Metabolism
ELISA:
1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.
Immunocytochemistry:
1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).
Western blot:
1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.
Immunoaffinity purification of GAPDH.
Optimal working dilutions must be determined by end user.
Research Sub Category
Enzymes & Biochemistry
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
质量
Evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
目标描述
38 kDa
外形
Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
Format: Purified
Protein A purified
储存及稳定性
Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
分析说明
Control
It is expressed in all cells.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 6C5, monoclonal |
species reactivity | rabbit, feline, mouse, rat, fish, pig, canine, human |
should not react with | E. coli |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunocytochemistry: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NP_002037 |
UniProt登记号 | P04406 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich