产品说明
一般描述
4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis.
应用
4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods.
包装
5 g in glass bottle
基本信息
| 经验(实验)分子式 | C20H10Br2O5 |
| 分子量 | 490.10 |
| 颜色索引号 | 45370:1 |
| Beilstein | 57189 |
| EC 号 | 209-876-0 |
| MDL编号 | MFCD00005042 |
| PubChem化学物质编号 | 24852976 |
| NACRES | NA.47 |
产品性质
| 质量水平 | 100 |
| 测定 | >95% (TLC) |
| 形式 | powder |
| 组成 | Dye content, 95% |
| mp | 270-273 ℃ (lit.) |
| λmax | 450 nm |
| ε (extinction coefficient) | ≥5000 at 445-451 nm ≥50000 at 227-233 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| 储存温度 | room temp |
| SMILES string | Oc1ccc2c(Oc3c(Br)c(O)ccc3C24OC(=O)c5ccccc45)c1Br |
| InChI | 1S/C20H10Br2O5/c21-15-13(23)7-5-11-17(15)26-18-12(6-8-14(24)16(18)22)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H |
| InChI key | ZDTNHRWWURISAA-UHFFFAOYSA-N |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H301 |
| 预防措施声明 | P301 + P310 |
| 危险分类 | Acute Tox. 3 Oral |
| 储存分类代码 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
Sigma-Aldrich
m.cnreagent.com
