Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

产品说明

一般描述

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.
Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).

免疫原

2,2,7-trimethylguanosine

包装

0.5 ml in Plastic ampoule

应用

Affinity Purification (see application references)

Immunoprecipitation (15 µl beads/sample)

Immunoprecipitation (1-2 µg/ml; see application references)

Immunocytochemistry (use Cat. No. NA02)

Paraffin Sections (use Cat. No. NA02)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

分析说明

Positive Control
Heterogenous nuclear RNA (hnRNA)
Negative Control
Transfer RNA (tRNA)

其他说明

Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem.269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res.16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell42, 725.
Kramer, A., et al. 1984. Cell38, 299.
Lerner, M.R., et al. 1980. Nature283, 220.
Also recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5′ cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息