产品说明
一般描述
NeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.
特异性
Other homologies: Human (94% sequence homology).
This antibody recognizes the N-terminus of NeuN.
免疫原
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.
应用
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.
质量
Evaluated by Western Blot in mouse brain E16 tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.
目标描述
~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.
外形
Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain E16 tissue lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | chicken |
质量水平 | 200 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, mouse |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgY |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich