Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

产品名称：

产品介绍：

产品说明

一般描述

Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.

特异性

Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.

免疫原

synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).

应用

Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:

in immunoblotting and dot blot
in immunoprecipitation
in immunohistochemistry
in enzyme linked immunosorbent assay (ELISA)
in western blotting
in chromatin immunoprecipitation(ChiP)

生化/生理作用

Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

外形

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02096544
NACRES	NA.41

产品性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 55 kDa
species reactivity	mouse, human
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells microarray: suitable western blot: 1:20,000 using nuclear extract from human HeLa cells
UniProt登记号	Q92769
运输	dry ice
储存温度	−20℃
Gene Information	human ... HDAC2(3066) mouse ... Hdac2(15182)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable