产品说明
一般描述
The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.
Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.
特异性
By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).
免疫原
recombinant human LIS1 protein.
应用
Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.
生化/生理作用
The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.
LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD03457563 |
| PubChem化学物质编号 | 24896475 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | LIS1-338, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 46 kDa |
| species reactivity | chicken, human, zebrafish, mouse, bovine, rat |
| 浓度 | ~2 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable microarray: suitable western blot: 0.5-1 μg/mL using a rat brain cytosol preparation |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P43034 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| SMILES string | CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O |
| InChI | 1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+ |
| InChI key | ZQTAMPRZFOOEEP-AVQMFFATSA-N |
| Gene Information | human ... PAFAH1B1(5048) mouse ... Pafah1b1(18472) rat ... Pafah1b1(83572) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
