产品说明
一般描述
Immunoglobulin G (IgG) is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.
特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Swine IgG F(ab′)2 and Swine Serum. No reaction was seen against Swine IgG F(c) .
免疫原
Swine IgG F(ab′)2 fragment
应用
Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).(3)
物理属性
Antibody format: IgG
外形
Supplied in 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
重悬
Reconstitute with 1.0 mL deionized water (or equivalent).
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.46 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
偶联物 | peroxidase conjugate |
抗体形式 | affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
形式 | lyophilized powder |
species reactivity | swine |
technique(s) | immunohistochemistry: suitable indirect ELISA: suitable western blot: suitable |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H317 |
预防措施声明 | P280 |
危险分类 | Skin Sens. 1 |
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich