产品说明
一般描述
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.
免疫原
synthetic peptide corresponding to amino acids 1-13 of the N-terminal of mouse p21 activated kinase 3 (PAK3).
应用
Anti-PAK3 may be used at a concentration of 4 μg to immunoprecipitate PAK3 from 0.5-1 mg mouse NIH3T3 total cell lysate. It may be used at a concentration of 1-2 μg/mL for detection in total mouse NIH3T3 lysate by immunoblotting.
外形
Solution in 0.07 M Tris-glycine, pH 7.4, containing 30% glycerol and 0.035% sodium azide.
基本信息
| MDL编号 | MFCD01633961 |
| NACRES | NA.44 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous glycerol solution |
| 分子量 | antigen 65 kDa |
| species reactivity | mouse, rat |
| technique(s) | immunoprecipitation (IP): 4 μg using 0.5 mg of mouse brain cytosol western blot (chemiluminescent): 2 μg/mL using mouse 3T3 fibroblasts extract or mouse brain extract |
| UniProt登记号 | Q62829 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Pak3(18481) rat ... Pak3(29433) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter |
Sigma-Aldrich
m.cnreagent.com
