Anti-phospho-PKB (pSer⁴⁷³) antibody produced in rabbit

产品说明

一般描述

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr³⁰⁸ and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death

特异性

Anti-phospho PKB (pSer⁴⁷³) recognises PKB phosphorylated at serine 473 (56 kDa).

免疫原

Synthetic phosphorylated peptide corresponding to (pSer473) PKBα (human, amino acids 461-477 with N-terminally added lysine), conjugated to KLH. The sequence is identical in rat, mouse, viral Akt and bovine PKBα and is highly conserved in chicken PKBα-(single amino acid substitution). The immunogen sequence is conversed to a lesser degree in PKBγ (88%) and PKBβ (64%).

应用

Anti-phospho-PKB (pSer473) antibody produced in rabbit has been used in western blotting.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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