产品说明

一般描述

该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色，该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is an essential vitamin required by cells in living organisms or in cultures. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin.

免疫原

生物素-KLH偶联物

应用

该抗体可被用于扩增技术、ELISA、免疫印迹、原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
Monoclonal Anti-Biotin-FITC antibody produced in mouse has been used in the localization of the antigen with a primary antibody, addition of a biotinylated antibody to bind to primary antibody and finally application avidin-FITC.

生化/生理作用

An immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

外形

Solution in phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

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