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位置:首页 > 品牌 > Sigma-Aldrich > Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse

Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse

品牌
Sigma-Aldrich
货号
D0180
规格纯度
clone DDC-109, ascites fluid
参考价格
4731.57 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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产品名称:
Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse
产品介绍:

产品说明

一般描述

DDC is a 85-90 kDa molecular weight protein that is mainly expressed in brain, kidney and liver.
Monoclonal Anti-DOPA Decarboxylase (DDC) (mouse IgG2b isotype) is derived from the DDC-109 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

应用

Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse may also be used in immunoprecipitation, immunocytochemistry and ELISA applications. In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers. It is suitable for use in western blotting at a working dilution of 1:1000 using bovine brain extract. It was used for immunoblotting and immunoprecipitation of AADC (aromatic amino acid decarboxylase) from rat striatum in a study. It was used as a primary antibody for western blotting and co-immunoprecipitation studies using human embryonic kidney cells and human neuroblastoma cells.

生化/生理作用

DOPA Decarboxylase (DDC), which requires pyridoxal phosphate for its activity, decarboxylates 5-hydroxytryptophan to form serotonin. It also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic neurons.
DOPA decarboxylase (DDC, EC 4.1.1.28), catalyzes the decarboxylation of the 5-hydroxytrptophan (5-HTP) to 5-HT. It requires pyridoxal phosphate for its activity. This is the enzyme that also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic.. Antibodies that react specifically with the enzyme enable the identification of both noradrenergic, dopaminergic, and serotoninergic systems, since DDC is present and active in all three types of neurons.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162261
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆DDC-109, monoclonal
分子量antigen ~50 kDa
包含15 mM sodium azide
species reactivityhuman, rat, sheep, rabbit, monkey, bovine, canine, guinea pig
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using bovine brain extract
同位素/亚型IgG2b
UniProt登记号P20711
运输dry ice
储存温度−20℃
Gene Informationhuman ... DDC(1644)
rat ... Ddc(24311)

Sigma-Aldrich

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