产品说明

一般描述

Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.

特异性

识别CD57/HNK-1人骨髓细胞相关的表面糖蛋白。识别的表位是存在于各种糖蛋白和某些糖脂中的N-连接碳水化合物。它耐受福尔马林固定和石蜡包埋。VC1.1抗体和HNK-1(Leu7)抗体能够抑制彼此的结合。该抗体识别某些物种中的髓磷脂相关糖蛋白和高分子量硫酸软骨素蛋白聚糖。
第 5 次研讨会：代码NK 67

免疫原

猫初级视觉皮层(17区)的匀浆

应用

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:

• as primary antibody for western blot analysis
• immunocytochemistry
• immunocytochemical
• immunohistological staining

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

生化/生理作用

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

目标描述

CD57/HNK-1表达于15-20％的外周血单核细胞亚群、约60％的NK活性细胞以及T细胞亚群中。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164634
NACRES	NA.41

产品性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	VC1.1, monoclonal
形式	buffered aqueous solution
包含	15 mM sodium azide
species reactivity	rat, human, feline, mouse
technique(s)	western blot: 1:4,000 using cerebral cortex extract
同位素/亚型	IgM
UniProt登记号	Q9P2W7
运输	dry ice
储存温度	−20℃
Gene Information	human ... B3GAT1(27087) mouse ... B3gat1(76898) rat ... B3gat1(117108)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable