Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

产品说明

特异性

The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.

免疫原

human uterus smooth muscle extract.

应用

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:

• immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
• immunoprecipitation
• western blot at a dilution of 1:4,000 using human uterus extract

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

生化/生理作用

Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164554
NACRES	NA.41

产品性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	hHCD, monoclonal
包含	15 mM sodium azide
species reactivity	sheep, mouse, pig, human, rabbit, bovine
technique(s)	immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue immunoprecipitation (IP): suitable western blot: 1:4,000 using human uterus extract
同位素/亚型	IgG1
UniProt登记号	Q05682
运输	dry ice
储存温度	−20℃
Gene Information	human ... CALD1(800) mouse ... Cald1(109624)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable