产品说明
一般描述
SCF (stem cell factor) is a hematopoietic cytokine that exists in both soluble and transmembrane forms, which arise due to alternative splicing. Both these forms are functionally active. This gene is localized to human chromosome 12q22-12q24. Endothelial cells and fibroblasts constitutively produce both transmembrane and soluble forms of SCF. It is also produced by keratinocytes in normal skin, and epithelial cells in the gut, and thymus. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
生化/生理作用
SCF (stem cell factor) exerts its activity by signaling through the c-Kit receptor. They participate in hematopoiesis, and development of mast cells and melanocytes. Mutations in either of these proteins result in various phenotypic disorders, such as varying degrees of macrocytic anemia, suppressed numbers of tissue mast cells, reduced fertility, and decreased pigmentation. Linkage analysis along with whole-exome sequencing, c.286_303delinsT (p.Ser96Ter), shows a heterozygous truncating mutation in this gene linked with congenital and stable non-syndromic unilateral and asymmetric hearing loss. rs4590952 polymorphism in this gene is linked with disruption of p53 function through increase in p53-dependent SCF expression, which results in increased susceptibility to testicular cancer.
序列
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
外形
Lyophilized from 10 mM Acetic Acid.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20℃ to -80℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥98% (HPLC) ≥98% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | ≤2.0 ng/mL ED50 |
| 分子量 | 18.4 kDa |
| 包装 | pkg of 10 μg |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | <0.1 EU/μg endotoxin, tested |
| 颜色 | white |
| UniProt登记号 | P21583 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... KITLG(4254) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H315 - H319 |
| 预防措施声明 | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| 危险分类 | Eye Irrit. 2 - Skin Irrit. 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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