产品说明
一般描述
Stage-specific embryonic antigen-1 (SSEA-1) is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
特异性
This antibody recognizes mouse Stage-Specific Embryonic Antigen-1 (SSEA-1).
免疫原
F9 teratocarcinoma stem cells (X-irradiated).
应用
Detect SSEA1 using this Anti-SSEA1 Antibody, clone MC-480, Cy3 conjugate validated for use in ICC.
Research Sub Category
Pluripotent & Early Differentiation
Glycobiology
Research Category
Stem Cell Research
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected no SSEA-1 in H9 human embryonic stem cells.
质量
Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected SSEA-1 in mouse embryonic stem cells (SCR012).
目标描述
The unconjugated parent antibody (Catalog No. MAB4301) has an observed MW at 220-240 kDa
外形
Ammonium sulfate precipitation followed by size exclusion.
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
储存及稳定性
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
Mouse embryonic stem cells (SCR012).
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
偶联物 | CY3 conjugate |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | MC-480, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable |
同位素/亚型 | IgM |
NCBI登记号 | NP_002024 |
UniProt登记号 | P22083 |
运输 | wet ice |
Gene Information | human ... FUT4(2526) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich