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Tuner(DE3)pLysS Competent Cells-Novagen

品牌
Sigma-Aldrich
货号
70624
规格纯度
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
参考价格
2069.46 *本价格含增值税费
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数量
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产品名称:
Tuner(DE3)pLysS Competent Cells-Novagen
产品介绍:

产品说明

一般描述

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: FompT hsdSB (rB mB) gal dcm lacY1(DE3) pLysS (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

包装

0.4 ml in Plastic ampoule
1 ml in Glass bottle

组分

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
manufacturer/tradenameNovagen®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
细胞转化transformation efficiency: >2×106 cfu/μg
储存温度−70℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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