产品说明
一般描述
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
Tuner
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F–ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) pLysS (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
包装
0.4 ml in Plastic ampoule
1 ml in Glass bottle
组分
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
manufacturer/tradename | Novagen® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
细胞转化 | transformation efficiency: >2×106 cfu/μg |
储存温度 | −70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich