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Nova Taq DNA Polymerase

品牌
Sigma-Aldrich
货号
71003-M
规格纯度
Ultrapure recombinant enzyme for dependable PCR amplification
参考价格
892.75 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Nova Taq DNA Polymerase
产品介绍:

产品说明

一般描述

NovaTaq DNA Polymerase* is a premium quality, recombinant form of Thermus aquaticus DNA polymerase that is licensed for PCR. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible perfomance, each preparation is extensively tested in a variety of quality control assays. NovaTaq DNA Polymerase has an integral 5′→3′ exonuclease and lacks 3′→5′ exonuclease activity. The enzyme generates PCR products with 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.
Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration: 5.0 U/μl
Purity: 95% homogeneous by SDS-PAGE
Endonuclease: None detected
Amplification effiency: Functional PCR
Storage: –20℃
NovaTaq DNA polymerase is a recombinant form of Thermus aquaticus DNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaq DNA polymerase exhibits 5′-3′ DNA polymerase activity and lacks 3′- 5′ exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3′-dA overhangs, suitable for cloning with Novagen®Perfectly Blunt®, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.
Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration: 5.0 U/μl
Purity: 95% homogeneous by SDS-PAGE
Endonuclease: None detected
Amplification effiency: Functional PCR
Storage: –20℃

应用

NovaTaq DNA polymerase has been used to check the success of the ligation step during cloning. It has also been used in the PCR amplification of cDNA.
NovaTaq DNA Polymerase can be used in standard polymerase chain reaction (PCR) amplification protocols. It is also suitable for colony PCR for screening bacterial colonies.

特点和优势

  • Higher yield and PCR specificity
  • Improved low-copy target amplification
  • Amplification of target sequences up to 5 kb
  • Ambient temperature setup compatible with automation
  • Ideal for quantitative and high-throughput applications

组分

•100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase

•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl₂

•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer without MgCl₂

•1 or 2 or 7 × 1.5 ml25 mM MgCl₂

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

单位定义

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-32P]dCTP, and activated salmon sperm DNA.

法律信息

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
用途 sufficient for 2000 reactions
sufficient for 400 reactions
sufficient for 80 reactions
特点dNTPs included: no
hotstart: no
manufacturer/tradenameNovagen®
储存条件OK to freeze
technique(s)PCR: suitable
input purified DNA
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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