Anti-NG2, Alexa Fluor^®488 Conjugate Antibody

产品说明

一般描述

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

特异性

This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

免疫原

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat

应用

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NG2 in adult rat brain tissue.
Research Category
Neuroscience
Anti-NG2, Alexa Fluor^®488 Conjugate Antibody detects level of NG2 & has been published & validated for use in ICC & IHC.
Research Sub Category
Neurodegenerative Diseases

质量

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

目标描述

The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa

外形

Purified rabbit polyclonal conjugated to Alexa Fluor^® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A adsorption

储存及稳定性

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
Rat oligodendrocyte precursor cells

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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