产品说明

一般描述

Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.
Note: 4µg = 6000 units.

包装

4 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.

外形

In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息

象形图
警示用语：	Warning
危险声明	H317
预防措施声明	P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类	Skin Sens. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 2