Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

产品说明

一般描述

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

特异性

Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.
Predicted to cross-react with mouse and bovine based on sequence homology.

免疫原

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

应用

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.

质量

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.
Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

目标描述

150 kDa

联系

Replaces: 05-841

外形

Cultured supernatant containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
RIPA lysates from PC-12 cells

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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