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Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-119
规格纯度
culture supernatant, from rabbit
参考价格
6694.05 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal
H4K12Ac, Histone H4 (acetyl K12)
产品介绍:

产品说明

特异性

Recognizes Histone H4 when acetylated on Lys12.
Wide range of cross-reactivity expected based on sequence homology.

免疫原

Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.

应用

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
This Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).

质量

Routinely evaluated by immunoblot.

目标描述

11kDa

外形

100 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.

储存及稳定性

2 years at -20°C from date of shipment

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆monoclonal
species reactivityvertebrates, human
manufacturer/tradenameChemicon®
Upstate®
technique(s)ChIP: suitable
dot blot: suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_003538.3
UniProt登记号P62805
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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