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位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

品牌
Sigma-Aldrich
货号
04-797
规格纯度
clone Aw39, Upstate®, from rabbit
参考价格
5520.38 *本价格含增值税费
促销
服务
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数量
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产品名称:
Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39
Mitogen-activated protein kinase, Extracellular signal-regulated kinase, protein tyrosine kinase ERK2
产品介绍:

产品说明

一般描述

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).

特异性

Predicted to cross-react with many species based on sequence homology.
Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.

应用

Research Sub Category
MAP Kinases
Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).
Research Category
Signaling

质量

Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells

目标描述

MAPK1 (~44 kDa) and MAPK2 (~42 kDa).

联系

Replaces: 05-797

外形

Format: Purified
Cultured supernantant in 0.05% sodium azide

储存及稳定性

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
RIPA cell lysate from NGF stimulated PC-12 cells

其他说明

Concentration: Variable

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆Aw39, monoclonal
species reactivityhuman, rat
manufacturer/tradenameUpstate®
technique(s)multiplexing: suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_002745
NM_002746
UniProt登记号P28482
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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