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Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal

品牌
Sigma-Aldrich
货号
04-739
规格纯度
culture supernatant, clone AM223, from rabbit
参考价格
6022.61 *本价格含增值税费
促销
服务
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  • 增值税票
数量
-+
产品名称:
Anti-Raf-1 Antibody, clone AM223, rabbit monoclonal
Oncogene RAF1, raf proto-oncogene serine/threonine protein kinase, v-raf-1 murine leukemia viral oncogene homolog 1
产品介绍:

产品说明

一般描述

The Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a c-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. In addition to its role in mitogenesis, Raf-1 may play a role in regulation of apoptosis and cell cycle progression. Activation of Raf-1 involves phosphorylation of Ser338/339 and Tyr340/341. Activating mutations of B-Raf that disrupt its auto-inhibition loop have been implicated in a number of cancers, including melanoma and colon cancer.

特异性

Predicted to cross-react with rat based on sequence homology.
Recognizes Raf-1, Mr 74 kDa.

免疫原

KLH-conjugated, synthetic peptide corresponding to the C-terminus (amino acids 637-648 (CTLTTSPRLPVF)) of human Raf-1.
Epitope: C-terminus

应用

Research Sub Category
MAP Kinases
Anti-Raf-1 Antibody, clone AM223 is an antibody against Raf-1 for use in IP & WB.
Research Category
Signaling
Western Blotting Analysis:
A 1:500-1:2,000 diltuoin of this lot detected Raf-1 in 3T3/A31 cell lysates.
Immunoprecipitation: 2-4 μg of a previous lot immunoprecipitated Raf-1 from 400 μg of 3T3/A31 RIPA lysate.

质量

Routinely evaluated by Western Blot on 3T3/A31 lysates.

目标描述

74 kDa

联系

Replaces: 04-412

外形

Cultured supernatant with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
3T3/A31 cell lysate.
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆AM223, monoclonal
species reactivitymouse, human, rat
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_002880
UniProt登记号P04049
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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