Anti-Aldolase C Antibody, clone 3B18, ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Aldolase C. It targets an epitope within 22 amino acids from the C-terminal region.

免疫原

KLH-conjugated liner peptide corresponding to 22 amino acids from the C-terminal region of human Aldolase C.

应用

Anti-Aldolase C, clone 3B18 ZooMAb, Cat. No. ZRB1354, is a recombinant Rabbit monoclonal antibody that targets Aldolase C and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aldolase C in human brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Aldolase C in human cerebellum and human cerebral cortex tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Aldolase C in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Fructose-bisphosphate aldolase C (UniProt: P09972; also known as EC:4.1.2.13, Brain-type aldolase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID: 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible conversion of fructose 1-6-diphosphate to glyceraldehyde 3-phosphate and dihydroxy-acetone phosphate via the glycolytic pathway. Within the cell, aldolase is localized in both the cytoplasm and nucleus. In vertebrates, three forms of this aldolases have been described, aldolase A in muscle, aldolase B in liver, and aldolase C in brain. Aldolase C is a homotetrameric enzyme that is expressed mainly in cerebellum and retina. Its kinetic properties are somewhat intermediate between those of the A and B isoforms. In fetal tissues only low levels of aldolase C have been detected. It serves as a useful marker for the study of cerebellar compartmentalization, due to its specific expression in distinct subpopulations of cerebellar Purkinje cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fujita. H., et al. (2014). PLoS ONE 9(1): e86679; Waltheer, EU., et al. (1998). Proc. Natl. Acad. Sci. USA. 95(5); 2615-2620).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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