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位置:首页 > 品牌 > Sigma-Aldrich > Anti-CACNA1C Antibody, clone 1F20 ZooMAb® Rabbit Monoclonal

Anti-CACNA1C Antibody, clone 1F20 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1293
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-CACNA1C Antibody, clone 1F20 ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1F20 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Calcium Channel- 1C Subunit (CACNA1C). It targets an epitope within 17 amino acids from the 7th cytoplasmic domain within the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the 7th cytoplasmic domain of human Voltage-dependent L-type calcium channel subunit a-1C (CACNA1C).

应用

Anti-CACNA1C, clone 1F20 ZooMAb®, Cat. No. ZRB1293, is a recombinant Rabbit monoclonal antibody that specifically targets CACNA1C and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Human Heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CACNA1C in Human Heart tissue lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CACNA1C in L6 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CACNA1C in human heart and human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Voltage-dependent L-type calcium channel subunit alpha-1C (UniProt: Q13936; also known as Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle Voltage-gated calcium channel subunit alpha Cav1.2, CACNA1C) is encoded by the CACNA1C (also known as CACH2, CACN2, CACNl1A1, CCHL1A1) gene (Gene ID: 775) in human. CACNA1C is a multi-pass membrane protein that serves as a pore-forming alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. It colocalizes with ryanodine receptors in distinct clusters at the junctional membrane, where the sarcolemma and the sarcoplasmic reticulum are in close contact. It mediates the influx of calcium ions into the cytoplasm, and thereby triggers calcium release from the sarcoplasm. It is also reported to play a role in excitation-contraction coupling in the heart and is required for normal heart development and normal regulation of cardiac rhythm. Its phosphorylation by protein kinase A at seine 1981 is shown to activate the channel. CACNA1C is also shown to be essential for normal contraction of smooth muscle cells in blood vessels that is required for normal blood pressure regulation. Its activity can be inhibited by treatment with nifedipine and dihydropyrdines. Mutations in CACNa1C gene are known to cause long QT syndrome that is characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源rabbit
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1F20, recombinant monoclonal
描述recombinant, expressed in HEK 293 cells
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 248.98 kDa
observed mol wt ~250 kDa
species reactivityhuman, rat
species reactivity (predicted by homology)monkey, mouse, bovine
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
表位序列N-terminus
Protein ID登记号NP_955630
UniProt登记号Q13936
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

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