产品说明
一般描述
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特异性
Clone 3H17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Dickkopf-related protein 3 (DKK3).
免疫原
His-tagged recombinant fragment corresponding to 300 amino acids (almost full length mature protein) from human Dickkopf-related protein 3 (DKK3).
应用
Affinity Binding Assay: A representative lot of this antibody bound recombinant DKK3 protein with a KD of 5.7 x 10-9 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in MCF-7 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected DKK3 in one million MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-DKK3, clone 3H17 ZooMAb, Cat. No. ZRB1074, is a recombinant Rabbit monoclonal antibody that targets DKK3 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
目标描述
Dickkopf-related protein 3 (UniProt: Q9UBP4; also known as Dickkopf-3, Dkk-3, hDkk-3) is encoded by the DKK3 (also known as REIC, UNQ258/PRO295) gene (Gene ID: 27122) in human. DKK is a family of secreted glycoproteins that display Wnt inhibitory activity. They act as high affinity ligands for the transmembrane proteins Kremen1 and 2, which are known to modulate Wnt signaling. They play a role in vertebrate development via locally inhibiting Wnt-regulated processes such as antero posterior axial patterning, limb development, somitogenesis and eye formation. DKK3 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. High expression of DKK3 is reported in heart, brain, and spinal cord. DKK3 is shown to antagonize canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with Kremen protein, which is known to promote internalization of LRP5/6. The C-terminal cysteine-rich domain of DKK3 mediates its interaction with LRP5 and LRP6. DKK3 is shown to induce a redistribution of β-catenin to the cell membrane where β-catenin promotes cell-cell adhesion by binding to the cytoplasmic domain of E-cadherin to form adheren junction complex. This binding also prevents nuclear translocation of β-catenin that reduces Wnt-mediated LEF/TCF transactivation. DKK3 is proposed to act as a tumor suppressor protein and its over-expression is shown to suppress tumor growth. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoang, BH., et al. (2004). Cancer Res. 64(8); 2734-2339; Niehrs. C. (2006). Oncogene. 25(57); 7469-7481).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
生物来源 | rabbit |
质量水平 | 200 |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 3H17, monoclonal recombinant monoclonal |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 38.39 kDa observed mol wt ~58 kDa |
species reactivity | human |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | affinity binding assay: suitable immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich