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位置:首页 > 品牌 > Sigma-Aldrich > Anti-DKK3 Antibody, clone 3H17 ZooMAb® Rabbit Monoclonal

Anti-DKK3 Antibody, clone 3H17 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1074
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-DKK3 Antibody, clone 3H17 ZooMAb® Rabbit Monoclonal
Dkk-3
hDkk-3
Dickkopf-3
Dickkopf-related protein 3
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 3H17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Dickkopf-related protein 3 (DKK3).

免疫原

His-tagged recombinant fragment corresponding to 300 amino acids (almost full length mature protein) from human Dickkopf-related protein 3 (DKK3).

应用

Affinity Binding Assay: A representative lot of this antibody bound recombinant DKK3 protein with a KD of 5.7 x 10-9 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in MCF-7 cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected DKK3 in one million MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected DKK3 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-DKK3, clone 3H17 ZooMAb, Cat. No. ZRB1074, is a recombinant Rabbit monoclonal antibody that targets DKK3 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Dickkopf-related protein 3 (UniProt: Q9UBP4; also known as Dickkopf-3, Dkk-3, hDkk-3) is encoded by the DKK3 (also known as REIC, UNQ258/PRO295) gene (Gene ID: 27122) in human. DKK is a family of secreted glycoproteins that display Wnt inhibitory activity. They act as high affinity ligands for the transmembrane proteins Kremen1 and 2, which are known to modulate Wnt signaling. They play a role in vertebrate development via locally inhibiting Wnt-regulated processes such as antero posterior axial patterning, limb development, somitogenesis and eye formation. DKK3 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. High expression of DKK3 is reported in heart, brain, and spinal cord. DKK3 is shown to antagonize canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with Kremen protein, which is known to promote internalization of LRP5/6. The C-terminal cysteine-rich domain of DKK3 mediates its interaction with LRP5 and LRP6. DKK3 is shown to induce a redistribution of β-catenin to the cell membrane where β-catenin promotes cell-cell adhesion by binding to the cytoplasmic domain of E-cadherin to form adheren junction complex. This binding also prevents nuclear translocation of β-catenin that reduces Wnt-mediated LEF/TCF transactivation. DKK3 is proposed to act as a tumor suppressor protein and its over-expression is shown to suppress tumor growth. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hoang, BH., et al. (2004). Cancer Res. 64(8); 2734-2339; Niehrs. C. (2006). Oncogene. 25(57); 7469-7481).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3H17, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 38.39 kDa
observed mol wt ~58 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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