产品说明
一般描述
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特异性
Clone 2C18 is a Rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 2A (eIF-2A). It targets an epitope within 23 amino acids from the C-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human Eukaryotic translation initiation factor 2A (eIF-2A).
应用
Quality Control Testing
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF2A in PC12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF2A in mouse pancreas tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF2A in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF2A in U2OS cells..
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
目标描述
Eukaryotic translation initiation factor 2A (UniProt: Q9BY44; also known as eIF-2A, 65 kDa eukaryotic translation initiation factor 2A) is encoded by the EIF2A (also known as CDA02, MSTP004, MSTP089) gene (Gene ID: 83939) in human. eIF-2A is a widely expressed protein with higher levels observed in pancreas, heart, brain, and placenta. It functions in the early steps of protein synthesis by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. It is reported to catalyze the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low magnesium levels. It has seven WD repeats. Although both eIF2 and eIF-2A function in the methionyl-puromycin assays, the order of events differs between the two. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. Note: The gene EIF2A should not be confused with EIF2S1 (Gene ID: 1965) that is frequently referred to as eIF2-alpha in the literature. EIF2S1 is the alpha subunit of the eIF2 translation initiator complex. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zoll, WL., et al. (2002). J. Biol. Chem. 277(40); 37079-37087).
外形
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重悬
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.
法律信息
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
质量水平 | 200 |
生物来源 | rabbit |
重组 | expressed in HEK 293 cells |
偶联物 | unconjugated |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2C18, recombinant monoclonal |
描述 | recombinant, expressed in HEK 293 cells |
产品线 | ZooMAb® learn more |
形式 | lyophilized |
分子量 | calculated mol wt 64.99 kDa observed mol wt ~60 kDa |
纯化方式 | using Protein A |
species reactivity | human, rat, mouse |
species reactivity (predicted by homology) | monkey, canine |
包装 | antibody small pack of 25 μL |
环保替代产品特性 | Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry. |
增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG |
表位序列 | C-terminal half |
Protein ID登记号 | NP_114414 |
UniProt登记号 | Q9BY44 |
环保替代产品分类 | Aligned |
运输 | ambient |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich